Enzymatic condensation of acetate to acetoacetate in liver extracts.
نویسندگان
چکیده
* The trichloroacetic acid extracts were first acidified with sulfuric acid3 and kept overnight at 38” for decarboxylation of acetoacetate. The fluid was then neutralized with sodium hydroxide and phosphate, and the acetone distilled by the procedure of Folin.4 Acetone was determined calorimetrically by the method of Behre.6 Analogous results were obtained with the determination procedure of Lester and Greenberg.3
منابع مشابه
The mechanism of acetoacetate synthesis.
The observation of a coenayme A (CoA)-catalyzed acetoacetate synthesis from acetate and adenosinetriphosphate in pigeon liver extracts (1) grouped this reaction with the larger family of acetylation reactions. Furthermore it furnished partial confirmation of an earlier calculation (2) showing that 16,000 calories are required for the condensation of two acetates to acetoacetate. Thus, one energ...
متن کاملEnzymatic synthesis of citric acid. I. Synthesis with soluble enzymes.
The synthesis of citrate from acetate, ATP,’ and oxalacetate by soluble enzyme preparations from pigeon liver was briefly reported in 1949 (1). CoA was found to be required for activity. Similar results were later reported by Novelli and Lipmann (2) with preparations from yeast and Escherichiu coli, and by Elliott and Kalnitsky (3) with kidney acetone powder extracts. It was further shown (1) t...
متن کاملP-TSA catalyzed facile and efficient synthesis of polyhydroquinoline derivatives through hantzsch multi-component condensation.
A facile and efficient synthetic route to polyhydroquinolines has been developed via four-component condensation reactions of aldehydes, dimedone, ethyl acetoacetate and ammonium acetate in the presence of p-toluenesulfonic acid (p-TSA) catalyst in ethanol at ambient temperature through Hantzsch reaction. Simple work-up procedure, environmentally friendly, inexpensive and non-toxic catalyst, sh...
متن کاملWaugh Type Enneamolybdomanganate(IV) Catalyzed Synthesis of Polyhydrquinoline Through Hantzsch Multi-Component Condensation
An efficient one pot coupling of aldehydes, dimedone, ammonium acetate and ethyl acetoacetate by using catalytic amount of enneamolybdomanganate(IV) is reported. Various polyhydroquinoline derivatives have been prepared in high yields and comparatively less reaction time.
متن کاملSynthesis of N-substituted acridinediones and polyhydroquinoline derivatives in refluxing water.
Acridinediones were synthesized by the one-pot Hantzsch condensation of an aromatic aldehyde, 5,5-dimethyl-1,3-cyclohexanedione, and aniline/4-methylaniline in refluxing water. This method has then been extended to the four-component reaction of an aromatic aldehyde, 5,5-dimethyl-1,3-cyclohexanedione, ethyl acetoacetate and ammonium acetate for the synthesis of polyhydroquinoline derivatives. Th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Journal of biological chemistry
دوره 175 2 شماره
صفحات -
تاریخ انتشار 1948